Journal Basic Info

  • Impact Factor: 1.995**
  • H-Index: 8
  • ISSN: 2474-1647
  • DOI: 10.25107/2474-1647
**Impact Factor calculated based on Google Scholar Citations. Please contact us for any more details.

Major Scope

  •  Colon and Rectal Surgery
  •  Breast Surgery
  •  Gynecological Surgery
  •  Orthopaedic Surgery
  •  Minimally Invasive Surgery
  •  Transplant Surgery
  •  Urology
  •  Pediatric Surgery

Abstract

Citation: Clin Surg. 2016;1(1):1162.Research Article | Open Access

Radiosensitization of HT-29 Colorectal Cells and Xenografts by the Nitric Oxide Donor JS-K

Renteria O, Gao X and Huerta S

Dallas VA Medical Center, Dallas Texas, USA
University of Texas Southwestern at Dallas, Texas, USA

*Correspondance to: Sergio Huerta 

 PDF  Full Text DOI: 10.25107/2474-1647.1162

Abstract

Background: Nitric oxide (NO) donors have demonstrated radiosensitizing activity against colorectal cancer in vitro and in vivo. JS-K is an NO donor that releases NO upon activation by glutathione. We evaluated the activity of JS-K in vitro and in vivo against HT-29 cells.Methods: Cell survival was assessed by colony forming assays in cells subjected to ionizing radiation (IR) [0 - 6 Gray (Gy)] with and without JS-K [0.5 μM]. Mice were inoculated with HT-29 cells and randomized to four treatments: (1) control, (2) IR [2 Gy X 5], (3) JS-K [20 μmol/Kg i.p.], (4) JS-K+ IR.Results: JS-K-treated cells were more sensitive to IR (SF-4 = 0.35) vs. untreated cells (SF-4 = 0.45) and (SF-6 = 0.21 vs. 0.29; respectively). Following 34 days of treatment, the JS-K+IR xenografts had a 9.4% reduction of tumor growth (p≤ 0.001) and at 40 days a 14.3% decrease compared to control (p≤ 0.0001). Western blot analysis showed an increase in cleaved PARP-1 and AIF with a concomitant decrease in Bcl-2.Conclusion: JS-K demonstrated antitumor activity against HT-29 cells in vitro and in vivo. Caspase and non-caspase mediated mechanisms of apoptosis were involved in this response.

Keywords

Nitric oxide donor; Rectal cancer; Complete pathological response

Cite the article

Renteria O, Gao X, Huerta S. Radiosensitization of HT-29 Colorectal Cells and Xenografts by the Nitric Oxide Donor JS-K. Clin Surg. 2016; 1: 1162.

Search Our Journal

Journal Indexed In

Articles in PubMed

RAF Kinase Inhibitory Protein Expression and Phosphorylation Profiles in Oral Cancers
 PubMed  PMC  PDF  Full Text
Automated Sagittal Craniosynostosis Classification from CT Images Using Transfer Learning
 PubMed  PMC  PDF  Full Text
View More...

Articles with Grants

Safety and Effectiveness of Enhanced Recovery after Surgery (ERAS) in Patients with Hepatocellular Carcinoma Undergoing Partial Hepatectomy: A Multicentre, Randomized, Controlled Clinical Study
 Abstract  PDF  Full Text
The Value of Hysteroscopy before the First IVF/ICSI Treatment Cycle in Patients with Endometrial Polyp
 Abstract  PDF  Full Text
View More...