1β and TNF-α as a Biomarker of Recurrence in Malignant Eyelid Tumours

Sayeed K, Kaur A, Bhasker SK and Pant AB

Department of Ophthalmology, King George’s Medical University, India
Department of Ophthalmology, Indian Institute of Toxicology and Research, India

^*Correspondance to: Kaur A 

 PDF  Full Text DOI: 10.25107/2474-1647.1047

Abstract

Aim: To establish a correlation between cytokine levels (IL-1β, TNF-α and IL-10) and establishing them as a biomarker of recurrence in malignant eyelid tumours.Method: Prospective observational cross-sectional case-control study of 38 consecutive cases of malignant eyelid tumours that underwent surgical treatment over a period of 18 months. 26 age and sex matched controls with other non-inflammatory, non- neoplastic eyelid disorders. The levels of the cytokines [IL-1β (Interleukin- 1β), TNF-α (tumour necrosis factor-α), IL-10 (Interleukin-10)] (in pg/ml) were determined by using ELISA Kit. Data was analyzed statistically.Result: On comparing the mean cytokine levels of the two groups, t-test revealed significantly higher levels of IL- 1β (16.39 ± 2.86 vs. 14.62 ± 3.72, t=2.17, p=0.034), TNF-α (19.32 ± 3.47 vs. 16.74 ± 4.45, t=2.61, p=0.011) and IL-10 (24.79 ± 5.87 vs. 21.61 ± 4.61, t=2.33, p=0.023) in cases. ANOVA revealed significantly different levels of IL- 1β (F=7.86, p<0.001), TNF-α (F=7.42, p<0.001) and IL- 10 (F=4.36, p=0.006) between tumour and it’s three adjacent tissues.Conclusion: Cytokine levels (IL-1β, TNF-α and IL-10) in the adjacent tissues beyond safety margins were normal and comparable to that in controls suggesting the margins to be tumour free. Highly significant lower levels of IL-1β and TNF-α in tissues beyond safety margins can be used as an important predictor for local recurrence of tumour.

Keywords

Malignant eyelid tumour; IL-1β, TNF-α; Recurrence

Cite the article

Sayeed K, Kaur A, Bhasker SK, Pant AB. IL-1β and TNF-α as a Biomarker of Recurrence in Malignant Eyelid Tumours. Clin Surg. 2016; 1: 1047.