Ma Tianying, Ran Tianfei, Ke Song, Qin Yinyin and Wang Min*
Department of Orthopedics, The Second Affiliated Hospital of Army Medical University, ChinaFulltext PDF
Objective: To study the in vitro and in vivo properties of titanium alloys coated with carbon-based nanofilms. Methods: The experiments with two circular flake test specimens of the same specification, made from carbon-based nanofilm coated titanium alloy and Co-Cr-Mo alloy commonly used in artificial joints, respectively, were performed in vitro and in vivo in animals. In vitro experiments were performed by co-culturing the two test specimens with standard strains of Staphylococcus aureus and Escherichia coli, and the antibacterial properties of the two test specimens were evaluated from inhibition zone size, scanning electron microscopy, fluorescence staining, and colony forming unit count; their cytotoxicity was evaluated by co-culturing them with rabbit mesenchymal stem cells. In vivo experiments were performed by implanting the two test specimens in the muscle tissue of experimental rabbits to evaluate their tissue compatibility properties from histology. Results: Both in vitro co-cultures of carbon-based nanofilm titanium alloy and Co-Cr-Mo alloy with Staphylococcus aureus and Escherichia coli, respectively, failed to form obvious inhibition zone. However, some biofilms were observed on the surface of Co-Cr-Mo alloys. Fewer bacteria adhered to the novel titanium alloy coated with carbon-based nanofilms can be observed by scanning electron microscopy and fluorescence staining techniques. Meanwhile, the colony forming unit count also showed that the titanium alloy coated with carbon-based nanofilms had less bacterial adhesion than the Co-Cr-Mo alloy. After co-culture of the two materials with rabbit mesenchymal stem cells, there was no significant difference in cell count, and both the cell counts showed no cytotoxicity. There are relatively few giant cells and better histocompatibility in tissues near the titanium alloy coated with carbon-based nanofilms in the in vivo experiment of animals. Conclusion: The novel titanium alloy coated with carbon-based nanofilms has stronger antibacterial activity in vitro and better histocompatibility in vivo than Co-Cr-Mo alloy.
Carbon-based nanofilm coated titanium alloy; Co-Cr-Mo alloy; Material properties
Tianying M, Tianfei R, Song K, Yinyin Q, Min W. The Antibacterial Activity Comparison of Carbon-Based Nanofilm Coated Titanium Alloy and Co-Cr-Mo Alloy. Clin Surg. 2022; 7: 3503..