Kyoungyul Lee1, Kyu Sang Lee2, Sumi Yun3, Seyoung Moon2, Kyung-Ah Hwang4 and Gheeyoung Choe2*
1Department of Pathology, Kangwon National University School of Medicine, Korea
2Department of Pathology, Seoul National University College of Medicine, Korea
3Department of Diagnostic Pathology, Samkwang Medical Laboratories, Korea
4Department of Research and Development, SML Genetree, Korea
Background: Glioblastoma Multiforme (GBM) is the most frequent brain tumor with a median overall survival of less than 2 years. Several studies indicate that most of GBM tumors are associated with the Human Cytomegalovirus (HCMV). Based on HCMV infection of GBM in recent studies, experimental therapies including antiviral medication and immunotherapy are emerging. However, the presence of HCMV in GBM is still controversial because a minority of studies failed to identify the evidence of HCMV in GBM until recently. The aim of the study is to find the evidence of HCMV in Korean GMB tumors. Method: We collected 98 cases of GBM from 2004 to 2012. To detect the low level expression of HCMV antigen and gene in GBM tumors, we performed immunohistochemistry and real-time PCR. We used two antibodies, HCMV pp65 and HCMV EA, for immunohistochemistry. Eighteen cases containing sufficient tumor cells and showing moderate intensity in at least one of pp65 and EA immunohistochemistry were chosen to implement real-time PCR. Result: The HCMV pp65 antigen was detected in 73/90 (81.1%) and HCMV EA antigen was found in 46/90 (51.1%) of GBM cases. Seventeen cases (18.9%) showed moderate intensity for pp65 antigen and 12 cases (13.3%) showed moderate intensity for EA antigen. In contrary to positive immunohistochemical expressions, real-time PCR failed to detect HCMV UL55 gene in selected 18 glioblastoma specimens. Conclusion: HCMV antigens were immunohistochemically expressed in Korean GBM cases, which was not confirmed by real-time PCR. The evidence of HCMV infection in Korean GBM tumors was uncertain
Lee K, Lee KS, Yun S, Moon S, Hwang K-A, Choe G. Conflicting Outcomes of Human Cytomegalovirus Infection Detected with Immunohistochemistry and Real-Time PCR in Korean Glioblastoma Patients. Clin Surg. 2021; 6: 3352..