He Xiao1#, Ye Jiang2#, DaxinZhan3#, Ping Chen1#, Deqiang Xu1, Xinghuan Wang1, Michael E Di Santo4 and Xinhua Zhang1*
1Department of Urology, Zhongnan Hospital of Wuhan University, China
2Department of Urology, People’s Hospital of Qichun County, China
3Department of Anesthesiology, Zhongnan Hospital of University, China
4Department of Surgery and Biomedical Sciences, Cooper Medical School of Rowan University, USA
#These authors contribute equally to this work
Increasing evidences showed that a huge number of miRNAs were abnormally expressed in bladder cancer tissues and played vital roles in tumorigenesis, progression and metastasis. The aim of our study was to identify the differential miRNAs expression between bladder cancer and normal bladder tissues by analyzing the high-throughput miRNA data downloaded from TCGA database. Additionally, we evaluated the prognostic values of the differentially expressed miRNAs and constructed a four-miRNA signature that could effectively predict patient survival. According to the cut-off criteria (P<0.05 and |log2FC>1.2), a total of 348 differentially expressed miRNAs were identified between bladder cancer tissues and matched normal tissues, including 264 up-regulated miRNAs and 84 down-regulated miRNAs. The Kaplan-Meier survival method revealed the prognostic function of the four miRNAs (miRNA-217, miRNA-378c, miR-33b and miRNA-615). Univariate and multivariate Cox regression analysis showed that the four-miRNA signature was an independent prognostic factor in bladder cancer. The functional enrichment analysis showed that the target genes of four miRNAs may be involved in various pathways related to cancer, including Notch signaling pathway, PI3K-Akt signaling pathway, p53 signaling pathway, cGMPPKG signaling pathway, FOXO and signaling pathway. Taken together, our study suggested that four-miRNA signature could be used as a prognostic marker in bladder cancer.
Bladder cancer; miRNA profiles; miRNA signature; Prognosis; TCGA; Bioinformatics analysis