Clin Surg | Volume 1, Issue 1 | Research Article | Open Access

Effects of Epidermal Growth Factor on Colonic Anastomoses: An Experimental Study

Güneş ME1*, Çakar E2, Bayrak S2, Deniz MM3 and Çağlıkülekçi M4

1Department of General Surgery, Istanbul Esenyurt University, Turkey
2Department of General Surgery, Istanbul Research and Training Hospital, Turkey
3Department of General Surgery, Bayrampasa State Hospital, Turkey
4Department of General Surgery, Yeniyuzyıl University, Turkey

*Correspondance to: Mehmet Emin Gunes 

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Purpose: This study investigated the effect of Epidermal Growth Factor (EGF) on reducing anastomotic leak and wound-related complications in rats following colonic anastomosis.Methods: Wistar-albino male rats of 14-16 weeks and weighing 200-250 grams. First, the rats were equally randomized into a control and an experimental group. Then, in each of these two groups three subgroups were formed, each consisting of five rats that were sacrificed on the postoperative 3rd, 7th and 21st days. Using an insulin injector, a 10 microgram/kg dose of EGF was administered to the rats in the experimental group, first submucosally on the anastomosis region during the operation, then intraperitoneal at the postoperative 12th, 24th and 48th hours.Results: Biomechanical, histopathological and clinical examinations were conducted to evaluate the wound healing in the subjects. The bursting pressure and tensile strength of anastomosis were measured in both the control and EGF-administered groups and a comparative analysis was performed. The results of both parameters were found to be significantly higher in the EGFadministered groups than in the control group. On the postoperative 3rd day, an anastomosis leak was observed in one rat from the control group and the bursting pressure was measured as 0 mmHg. In the EGF-administered groups; the histopathological examination of the wound on the postoperative 3rd day showed higher values of serum fibrin, fibrocytes and fibroblast. On the postoperative 7th day; the granulation tissue was further developed and the granulation distance increased and on the 21st day; the strength of the granulation tissue was higher.Conclusion: We conclude that administering EGF to subjects following colonic anastomosis has positive effects on wound healing.


Colon; Anastomosis; Epidermal growth factor; Leakage; Bursting pressure; Tensile strength


Gunes ME, Cakar E, Bayrak S, Deniz MM, Caglikulekci M. Effects of Epidermal Growth Factor on Colonic Anastomoses: An Experimental Study. Clin Surg. 2016; 1: 1128.

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